fopflash reporter vector Search Results


pgl4.14 (luc2/hygro) vector  (Promega)
90
Promega pgl4.14 (luc2/hygro) vector
Pgl4.14 (Luc2/Hygro) Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl4.14 (luc2/hygro) vector/product/Promega
Average 90 stars, based on 1 article reviews
pgl4.14 (luc2/hygro) vector - by Bioz Stars, 2026-03
90/100 stars
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m51 super 8x fop flash  (Addgene inc)
94
Addgene inc m51 super 8x fop flash
M51 Super 8x Fop Flash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m51 super 8x fop flash/product/Addgene inc
Average 94 stars, based on 1 article reviews
m51 super 8x fop flash - by Bioz Stars, 2026-03
94/100 stars
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fopflash-luc  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc fopflash-luc
Fopflash Luc, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fopflash-luc/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
fopflash-luc - by Bioz Stars, 2026-03
90/100 stars
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fopflash  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc fopflash
A. Ectopic KIAA1199 expression in HEK293 cells inhibits <t>the</t> <t>transcriptional</t> activity elicited by wild-type CTNNB1 or by a constitutively active form of the protein, CTNNB1-T41A. Cells were transiently transfected with either a TOPflash or a <t>FOPflash</t> reporter vector, together with an empty vector or an expression vector for KIAA1199 or TCF4-DN. TOP/FOP ratios are the mean ± SEM of triplicate experiments. B. Total and active CTNNB1 levels in SW480 cells expressing KIAA1199 (V5) and in empty vector-transfected (Dest) controls. The table shows fold changes (vs. controls) in the expression of both CTNNB1 forms induced by KIAA1199 expression based on quantification of band intensity relative to that of CDH1 or TUBB in the same lane. TUBB was used as loading control for cytoplasmic and nuclear fractions since it was detectable in these fractions of the SW480-V5 and Dest cell extracts under the same extraction conditions. Furthermore, TUBB has been reported to shuttle to the nucleus ( and references herein).
Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fopflash/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
fopflash - by Bioz Stars, 2026-03
90/100 stars
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m50 super 8x topflash  (Addgene inc)
96
Addgene inc m50 super 8x topflash
A. Ectopic KIAA1199 expression in HEK293 cells inhibits <t>the</t> <t>transcriptional</t> activity elicited by wild-type CTNNB1 or by a constitutively active form of the protein, CTNNB1-T41A. Cells were transiently transfected with either a TOPflash or a <t>FOPflash</t> reporter vector, together with an empty vector or an expression vector for KIAA1199 or TCF4-DN. TOP/FOP ratios are the mean ± SEM of triplicate experiments. B. Total and active CTNNB1 levels in SW480 cells expressing KIAA1199 (V5) and in empty vector-transfected (Dest) controls. The table shows fold changes (vs. controls) in the expression of both CTNNB1 forms induced by KIAA1199 expression based on quantification of band intensity relative to that of CDH1 or TUBB in the same lane. TUBB was used as loading control for cytoplasmic and nuclear fractions since it was detectable in these fractions of the SW480-V5 and Dest cell extracts under the same extraction conditions. Furthermore, TUBB has been reported to shuttle to the nucleus ( and references herein).
M50 Super 8x Topflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m50 super 8x topflash/product/Addgene inc
Average 96 stars, based on 1 article reviews
m50 super 8x topflash - by Bioz Stars, 2026-03
96/100 stars
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luciferase reporter plasmid topflash  (Millipore)
90
Millipore luciferase reporter plasmid topflash
A. Ectopic KIAA1199 expression in HEK293 cells inhibits <t>the</t> <t>transcriptional</t> activity elicited by wild-type CTNNB1 or by a constitutively active form of the protein, CTNNB1-T41A. Cells were transiently transfected with either a TOPflash or a <t>FOPflash</t> reporter vector, together with an empty vector or an expression vector for KIAA1199 or TCF4-DN. TOP/FOP ratios are the mean ± SEM of triplicate experiments. B. Total and active CTNNB1 levels in SW480 cells expressing KIAA1199 (V5) and in empty vector-transfected (Dest) controls. The table shows fold changes (vs. controls) in the expression of both CTNNB1 forms induced by KIAA1199 expression based on quantification of band intensity relative to that of CDH1 or TUBB in the same lane. TUBB was used as loading control for cytoplasmic and nuclear fractions since it was detectable in these fractions of the SW480-V5 and Dest cell extracts under the same extraction conditions. Furthermore, TUBB has been reported to shuttle to the nucleus ( and references herein).
Luciferase Reporter Plasmid Topflash, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luciferase reporter plasmid topflash/product/Millipore
Average 90 stars, based on 1 article reviews
luciferase reporter plasmid topflash - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


A. Ectopic KIAA1199 expression in HEK293 cells inhibits the transcriptional activity elicited by wild-type CTNNB1 or by a constitutively active form of the protein, CTNNB1-T41A. Cells were transiently transfected with either a TOPflash or a FOPflash reporter vector, together with an empty vector or an expression vector for KIAA1199 or TCF4-DN. TOP/FOP ratios are the mean ± SEM of triplicate experiments. B. Total and active CTNNB1 levels in SW480 cells expressing KIAA1199 (V5) and in empty vector-transfected (Dest) controls. The table shows fold changes (vs. controls) in the expression of both CTNNB1 forms induced by KIAA1199 expression based on quantification of band intensity relative to that of CDH1 or TUBB in the same lane. TUBB was used as loading control for cytoplasmic and nuclear fractions since it was detectable in these fractions of the SW480-V5 and Dest cell extracts under the same extraction conditions. Furthermore, TUBB has been reported to shuttle to the nucleus ( and references herein).

Journal: PLoS ONE

Article Title: Early Insights into the Function of KIAA1199, a Markedly Overexpressed Protein in Human Colorectal Tumors

doi: 10.1371/journal.pone.0069473

Figure Lengend Snippet: A. Ectopic KIAA1199 expression in HEK293 cells inhibits the transcriptional activity elicited by wild-type CTNNB1 or by a constitutively active form of the protein, CTNNB1-T41A. Cells were transiently transfected with either a TOPflash or a FOPflash reporter vector, together with an empty vector or an expression vector for KIAA1199 or TCF4-DN. TOP/FOP ratios are the mean ± SEM of triplicate experiments. B. Total and active CTNNB1 levels in SW480 cells expressing KIAA1199 (V5) and in empty vector-transfected (Dest) controls. The table shows fold changes (vs. controls) in the expression of both CTNNB1 forms induced by KIAA1199 expression based on quantification of band intensity relative to that of CDH1 or TUBB in the same lane. TUBB was used as loading control for cytoplasmic and nuclear fractions since it was detectable in these fractions of the SW480-V5 and Dest cell extracts under the same extraction conditions. Furthermore, TUBB has been reported to shuttle to the nucleus ( and references herein).

Article Snippet: CTNNB1 (beta-catenin)-dependent transcriptional activity was studied in HEK293 cells transfected with the TOPflash or FOPflash reporter vectors (Upstate Biotechnology), which contain a multimeric consensus TCF/LEF binding site and an inactive mutated homologue of this site, respectively.

Techniques: Expressing, Activity Assay, Transfection, Plasmid Preparation